Vraag
Gepost op: 3 juli 2019Bereiding Carnoy oplossing
Antwoord
Het is niet gemakkelijk om te beslissen welke samenstelling te prefereren is. Ik heb in de literatuur volgende informatie gevonden. Ik zou overleggen met de stomatoloog ! Voor de filtratie zal u wel moeten informeren naar chloroform bestendige membraanfilters.
2.1 Preparation of solutions
The Carnoy's solutions used in this investigation were prepared by the same trained pharmaceutical, by using 6‐ml absolute ethanol, 3‐ml chloroform, 1‐ml acetic acid and 1‐g FeCl3.6H2O (ferric chloride hexahydrate) to obtain an approximately 10‐ml final solution. To obtain Carnoy's solution without chloroform, the 3 ml of chloroform was replaced by 3‐ml absolute ethanol.
The solutions produced, as described above, have supersaturated concentration relative to the ferric chloride hexahydrate salt. We proceeded with the filtration of the samples using a 0.45‐μm syringe filter, which allowed to obtain solutions of Carnoy's with or without chloroform filtered. Thus, four types of solution were analyzed in the present study: unfiltered Carnoy's solution with chloroform, filtered Carnoy's solution with chloroform, unfiltered Carnoy's solution without chloroform, and filtered Carnoy's solution without chloroform.
5 CONCLUSIONS
The absence of chloroform in solution may affect the performance of the solution due to the reduction of viscosity and ethanol penetration in tissues. Overall, Carnoy's solutions are acidic, stable in the initial 7 days, and prone to suffer alterations in the formulation with chloroform after the seventh day (turbidity) as well as iron precipitation in the container when used without filtering.