Vraag
Gepost op: 9 november 2017Waarom moet een melatonine siroop in de koelkast bewaard worden. En hoelang is deze houdbaar?
Melatonin solutions are frequently used in human, animal, and in vitro research. Generally, fresh solutions are prepared, for fear of instability of melatonin in solution. We tested the high‐performance liquid chromatography (HPLC) stability of melatonin in aqueous solutions stored room temperature, 4°C, and ‐70°C for up to 6 months. Solutions were prepared in a laminar flow hood using sterile technique, directly into sterile, pyrogen‐free glass vacuum vials for storage. Different concentrations were tested (1.0–113.0 μg/ml). There was no loss of potency as assessed by HPLC, and the preparations remained sterile and pyrogen‐free. We conclude that melatonin solutions may be prepared in batches maintained in sterile, pyrogen‐free vials at 4°C or at ‐70°C until use within 6 months.
Antwoord
Melatonin solutions are frequently used in human, animal, and in vitro research. Generally, fresh solutions are prepared, for fear of instability of melatonin in solution. We tested the high‐performance liquid chromatography (HPLC) stability of melatonin in aqueous solutions stored room temperature, 4°C, and ‐70°C for up to 6 months. Solutions were prepared in a laminar flow hood using sterile technique, directly into sterile, pyrogen‐free glass vacuum vials for storage. Different concentrations were tested (1.0–113.0 μg/ml). There was no loss of potency as assessed by HPLC, and the preparations remained sterile and pyrogen‐free. We conclude that melatonin solutions may be prepared in batches maintained in sterile, pyrogen‐free vials at 4°C or at ‐70°C until use within 6 months.